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HA fusion proteins. A B C. 132 K. 90 K. 55 K. 43 K. 34 K. < OctA (FLAG®)- tagged fusion ... S. KOREA TOLL FREE: 00798.1.1.002.0297 ... The LightCycler® 480 System offers new tools for mutation scanning.. DNA from several KRAS mutant cell lines (MIA PaCa-2, SW480, NP9, ... Amplifications were performed for 35 cycles with an annealing temperature of 55ºC. ... reagent (20x) (Fluidigm Corporation), 0.12 µL DNA-free water, and 2.52 µL PCR product. ... The Light Cycler 480 Software v1.5 (Roche Diagnostics, Risch-Rotkreuz, .... To analyze results, use the system software linked to the real-time ... Prepare RT-PCR mixture with 2.8 μL of RNAse-free water, 10 μL of SYBR Green ... of amplification (15 s at 94°C, 20s at 55°C, and 10 s at 72°C) on LightCycler 480 (Roche).. by M Samardzija · 2014 · Cited by 24 — Investigative Ophthalmology & Visual Science August 2014, Vol.55, 5304-5313. ... All the other lines used in this study were free of the rd8 mutation. ... calculated using LightCycler480 software (Roche Diagnostics) using a calibrator sample.. Pkg of 25, clear shell/white well PCR plate for Roche LightCycler 480 ... Process sampling and testing to ensure that plates are free of DNase, RNase, and .... ... model was generated, center point values (pH 5.5, 55◦C) were used instead. ... The data collected was analyzed by Roche LightCycler 480 software (release .... by C Antúnez · 2011 · Cited by 23 — 800 adults, ages 55 to 90, to participate in the research—ap- proximately 200 ... Switzerland) by using the LightCycler 480 Probes Master kit according to the .... Results Interpretation Guide: Roche LightCycler 480 Instrument II Artus LC ... Close the instrument and start the LightCycler software application 4. ... 35 Minuten mit dem Standard 96-Well Format und um ca 55 Minuten mit der 384-Well Platte.. 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A6120) on a Roche Lightcycler 480 with a 96 well plate instrument using both standard and Fast thermalcycling conditions. ... Circulating Cell Free DNA (CCF DNA) was purifed from plasma using the Maxwell® RSC instrument ... GoTaq qPCR Master Mix Quick Protocol FB103PDF (55 KB) – English.. Roche Applied Science. LightCycler® 480 Instrument. Operator's Manual. 16pt/20pt wenig Text. Imago medium. Software. Version 1.0 .... Labcyte Echo 525 or 55X Liquid Handler ... Roche LightCycler 480 or 1536 ... Tempo™ Automation Control Software to adapt Echo software application .... ... Ljgln22 mutant genotypes showed a relative water content of about 55% (see Díaz ... analysis, total RNA was treated with the TURBO DNA-free DNase (Ambion). ... in a LightCycler 480 thermal cycler (Roche) using a SensiFAST SYBR No-ROX ... of gene probesets the Pathexpress software was used (Goffard and Weiller, .... by T Maricic · 2020 · Cited by 7 — Notably, the extraction-free NEB assay uses 20-fold less mouthwash per assay ... z480 Analyzer and analyzed with Roche LightCycler 480 Software. ... RT-qPCR program for the NEB reaction was: 55°C for 4 minutes, 60°C for .... Data File Names Variable Name. SAS Label. LBDR54. HPV type 54. LBDR55. HPV type ... Nuclease-free water is used as negative control for the assay. The water ... 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Roche LightCycle 480 system software version 1.5 advanced relative quantification .... ... using LightCycler 480 qPCR instrument and LightCycler 480 Software 1.5.0 (Roche ... USA), 2.5 μM of both forward and reverse primer and nuclease free water. ... followed by 45 cycles of denaturation at 95◦C for 10 s, annealing at 55◦C .... Feb 22, 2021 — Color Compensation for the LightCycler® 480 II Instruments. ... software to subtract fluorescence crosstalk from a reporter dye into inappropriate ... Regularly decontaminate equipment and benches with ethanol-free decontaminant. ... Transcription. 55°C none. 5 min. 4.4. Initial. Denaturation. 95°C none.. by S Kullmann · 2009 · Cited by 18 — We investigated whether the EPHX2 K55R polymorphism, previously linked ... conducted by means of the LightCycler 480 software according to the ... by chi-square test using a free program http://statpages.org/ctab2x2.html.. 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